Human neutralizing Fab molecules against severe acute respiratory syndrome coronavirus generated by phage display.
Identifieur interne : 003E71 ( Main/Exploration ); précédent : 003E70; suivant : 003E72Human neutralizing Fab molecules against severe acute respiratory syndrome coronavirus generated by phage display.
Auteurs : Xiaoping Kang [République populaire de Chine] ; Bao-An Yang ; Yuyang Hu ; Hui Zhao ; Wei Xiong ; Yinhui Yang ; Bingyin Si ; Qingyu ZhuSource :
- Clinical and vaccine immunology : CVI [ 1556-6811 ] ; 2006.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (génétique), Anticorps antiviraux (immunologie), Anticorps antiviraux (isolement et purification), Banque de peptides, Données de séquences moléculaires, Facteurs immunologiques (), Facteurs immunologiques (immunologie), Fragments Fab d'immunoglobuline (génétique), Fragments Fab d'immunoglobuline (immunologie), Fragments Fab d'immunoglobuline (isolement et purification), Humains, RT-PCR, Région variable d'immunoglobuline (immunologie), Syndrome respiratoire aigu sévère (immunologie), Séquence d'acides aminés, Tests de sensibilité microbienne, Virus du SRAS (immunologie).
- MESH :
- génétique : Anticorps antiviraux, Fragments Fab d'immunoglobuline.
- immunologie : Anticorps antiviraux, Facteurs immunologiques, Fragments Fab d'immunoglobuline, Région variable d'immunoglobuline, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Anticorps antiviraux, Fragments Fab d'immunoglobuline.
- Banque de peptides, Données de séquences moléculaires, Facteurs immunologiques, Humains, RT-PCR, Séquence d'acides aminés, Tests de sensibilité microbienne.
English descriptors
- KwdEn :
- Amino Acid Sequence, Antibodies, Viral (genetics), Antibodies, Viral (immunology), Antibodies, Viral (isolation & purification), Humans, Immunoglobulin Fab Fragments (genetics), Immunoglobulin Fab Fragments (immunology), Immunoglobulin Fab Fragments (isolation & purification), Immunoglobulin Variable Region (immunology), Immunologic Factors (chemistry), Immunologic Factors (immunology), Microbial Sensitivity Tests, Molecular Sequence Data, Peptide Library, Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , chemistry : Immunologic Factors.
- chemical , genetics : Antibodies, Viral, Immunoglobulin Fab Fragments.
- chemical , immunology : Antibodies, Viral, Immunoglobulin Fab Fragments, Immunoglobulin Variable Region, Immunologic Factors.
- chemical , isolation & purification : Antibodies, Viral, Immunoglobulin Fab Fragments.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Peptide Library, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Human recombinant Fab fragments specific for the spike protein of severe acute respiratory syndrome coronavirus (SARS-CoV) were screened from a human Fab library, which was generated from RNAs from peripheral lymphocytes of convalescent SARS patients. Among 50 randomly picked clones, 12 Fabs specially reacted with S protein by an enzyme-linked immunosorbent assay. The microneutralizing test showed that one clone, designated M1A, had neutralizing activity on Vero E6 cells against SARS-CoV. DNA sequence analysis indicated that the light- and heavy-chain genes of M1A Fab belong to the kappa2a and 4f families, respectively. A neutralizing test on purified M1A demonstrated that 0.5 mg/ml of M1A completely inhibited SARS-CoV activity, with an absence of cytopathic effect for 7 days. Real-time fluorescence reverse transcription-PCR also proved the neutralizing capacity of M1A. These data showed that the number of virus copies was significantly reduced in the M1A-treated group, suggesting an important role for M1A in passive immunoprophylaxis against the SARS virus.
DOI: 10.1128/CVI.00037-06
PubMed: 16893997
Affiliations:
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Le document en format XML
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Yang</name></author><author><name sortKey="Hu, Yuyang" sort="Hu, Yuyang" uniqKey="Hu Y" first="Yuyang" last="Hu">Yuyang Hu</name></author><author><name sortKey="Zhao, Hui" sort="Zhao, Hui" uniqKey="Zhao H" first="Hui" last="Zhao">Hui Zhao</name></author><author><name sortKey="Xiong, Wei" sort="Xiong, Wei" uniqKey="Xiong W" first="Wei" last="Xiong">Wei Xiong</name></author><author><name sortKey="Yang, Yinhui" sort="Yang, Yinhui" uniqKey="Yang Y" first="Yinhui" last="Yang">Yinhui Yang</name></author><author><name sortKey="Si, Bingyin" sort="Si, Bingyin" uniqKey="Si B" first="Bingyin" last="Si">Bingyin Si</name></author><author><name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16893997</idno><idno type="pmid">16893997</idno><idno type="doi">10.1128/CVI.00037-06</idno><idno 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display.</title><author><name sortKey="Kang, Xiaoping" sort="Kang, Xiaoping" uniqKey="Kang X" first="Xiaoping" last="Kang">Xiaoping Kang</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yang, Bao An" sort="Yang, Bao An" uniqKey="Yang B" first="Bao-An" last="Yang">Bao-An Yang</name></author><author><name sortKey="Hu, Yuyang" sort="Hu, Yuyang" uniqKey="Hu Y" first="Yuyang" last="Hu">Yuyang Hu</name></author><author><name sortKey="Zhao, Hui" sort="Zhao, Hui" uniqKey="Zhao H" first="Hui" last="Zhao">Hui Zhao</name></author><author><name sortKey="Xiong, Wei" sort="Xiong, Wei" uniqKey="Xiong W" first="Wei" last="Xiong">Wei Xiong</name></author><author><name sortKey="Yang, Yinhui" sort="Yang, Yinhui" uniqKey="Yang Y" first="Yinhui" last="Yang">Yinhui Yang</name></author><author><name sortKey="Si, Bingyin" sort="Si, Bingyin" uniqKey="Si B" first="Bingyin" last="Si">Bingyin Si</name></author><author><name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name></author></analytic><series><title level="j">Clinical and vaccine immunology : CVI</title><idno type="ISSN">1556-6811</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Antibodies, Viral (genetics)</term><term>Antibodies, Viral (immunology)</term><term>Antibodies, Viral (isolation & purification)</term><term>Humans</term><term>Immunoglobulin Fab Fragments (genetics)</term><term>Immunoglobulin Fab Fragments (immunology)</term><term>Immunoglobulin Fab Fragments (isolation & purification)</term><term>Immunoglobulin Variable Region (immunology)</term><term>Immunologic Factors (chemistry)</term><term>Immunologic Factors (immunology)</term><term>Microbial Sensitivity Tests</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (génétique)</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps antiviraux (isolement et purification)</term><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Facteurs immunologiques ()</term><term>Facteurs immunologiques (immunologie)</term><term>Fragments Fab d'immunoglobuline (génétique)</term><term>Fragments Fab d'immunoglobuline (immunologie)</term><term>Fragments Fab d'immunoglobuline (isolement et purification)</term><term>Humains</term><term>RT-PCR</term><term>Région variable d'immunoglobuline (immunologie)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Séquence d'acides aminés</term><term>Tests de sensibilité microbienne</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Immunologic Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin Fab Fragments</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin Fab Fragments</term><term>Immunoglobulin Variable Region</term><term>Immunologic Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin Fab Fragments</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Anticorps antiviraux</term><term>Fragments Fab d'immunoglobuline</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Facteurs immunologiques</term><term>Fragments Fab d'immunoglobuline</term><term>Région variable d'immunoglobuline</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antiviraux</term><term>Fragments Fab d'immunoglobuline</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Humans</term><term>Microbial Sensitivity Tests</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Facteurs immunologiques</term><term>Humains</term><term>RT-PCR</term><term>Séquence d'acides aminés</term><term>Tests de sensibilité microbienne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human recombinant Fab fragments specific for the spike protein of severe acute respiratory syndrome coronavirus (SARS-CoV) were screened from a human Fab library, which was generated from RNAs from peripheral lymphocytes of convalescent SARS patients. Among 50 randomly picked clones, 12 Fabs specially reacted with S protein by an enzyme-linked immunosorbent assay. The microneutralizing test showed that one clone, designated M1A, had neutralizing activity on Vero E6 cells against SARS-CoV. DNA sequence analysis indicated that the light- and heavy-chain genes of M1A Fab belong to the kappa2a and 4f families, respectively. A neutralizing test on purified M1A demonstrated that 0.5 mg/ml of M1A completely inhibited SARS-CoV activity, with an absence of cytopathic effect for 7 days. Real-time fluorescence reverse transcription-PCR also proved the neutralizing capacity of M1A. These data showed that the number of virus copies was significantly reduced in the M1A-treated group, suggesting an important role for M1A in passive immunoprophylaxis against the SARS virus.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Hu, Yuyang" sort="Hu, Yuyang" uniqKey="Hu Y" first="Yuyang" last="Hu">Yuyang Hu</name><name sortKey="Si, Bingyin" sort="Si, Bingyin" uniqKey="Si B" first="Bingyin" last="Si">Bingyin Si</name><name sortKey="Xiong, Wei" sort="Xiong, Wei" uniqKey="Xiong W" first="Wei" last="Xiong">Wei Xiong</name><name sortKey="Yang, Bao An" sort="Yang, Bao An" uniqKey="Yang B" first="Bao-An" last="Yang">Bao-An Yang</name><name sortKey="Yang, Yinhui" sort="Yang, Yinhui" uniqKey="Yang Y" first="Yinhui" last="Yang">Yinhui Yang</name><name sortKey="Zhao, Hui" sort="Zhao, Hui" uniqKey="Zhao H" first="Hui" last="Zhao">Hui Zhao</name><name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Kang, Xiaoping" sort="Kang, Xiaoping" uniqKey="Kang X" first="Xiaoping" last="Kang">Xiaoping Kang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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